University of Leicester
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<b>Split-APEX implicates splicing factor SRSF1 and splicing helicases in ribosomal biogenesis</b>

dataset
posted on 2025-09-26, 12:16 authored by Ian EperonIan Eperon, Vasileios PaschalisVasileios Paschalis
<p dir="ltr">Scaffold files following mass spectrometry of proteins isolated on streptavidin beads following proximity biotinylation. This was done using (1 file) full-length APEX2 fused to the N-terminus of SRSF1 and (1 file) split-APEX, with complementary portions of APEX2 fused to the N-terminus of SRSF1 and to the N-terminus of helicases involved in pre-mRNA splicing. Experiments were done in triplicate.</p>

Funding

Strategic Longer and Larger Grant: Frontier Bioscience from the Biotechnology and Biological Sciences Research Council, BBSRC (How do RNA-binding proteins control splice site selection? BB/T000627/1)

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    Molecular & Cell Biology

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