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A Low-Cost Method of Skin Swabbing for the Collection of DNA Samples from Small Laboratory Fish.

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posted on 2016-11-07, 17:24 authored by Carl Breacker, Iain Barber, William H. J. Norton, Jonathan R. McDearmid, Ceinwen A. Tilley
Fin clipping of live fish under anesthesia is widely used to collect samples for DNA extraction. An alternative, potentially less invasive, approach involves obtaining samples by swabbing the skin of nonanesthetized fish. However, this method has yet to be widely adopted for use in laboratory studies in the biological and biomedical sciences. Here, we compare DNA samples from zebrafish Danio rerio and three-spined sticklebacks Gasterosteus aculeatus collected via fin clipping and skin swabbing techniques, and test a range of DNA extraction methods, including commercially available kits and a lower-cost, in-house method. We verify the method for polymerase chain reaction analysis, and examine the potential risk of cross contamination between individual fish that are netted together. We show that swabbing, which may not require the use of anesthesia or analgesics, offers a reliable alternative to fin clipping. Further work is now required to determine the relative effects of fin clipping and swabbing on the stress responses and subsequent health of fish, and hence the potential of swabbing as a refinement to existing DNA sampling procedures.

Funding

This project was supported in part by a BBSRC grant to JM (BB/N010140/1).

History

Citation

Zebrafish, Volume 13, Number 6, 2016

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Neuroscience, Psychology and Behaviour

Version

  • VoR (Version of Record)

Published in

Zebrafish

Publisher

Mary Ann Liebert

issn

1545-8547

eissn

1557-8542

Copyright date

2016

Available date

2016-11-07

Publisher version

http://online.liebertpub.com/doi/10.1089/zeb.2016.1348

Notes

All work was undertaken under the authority of a UK Home Office project licence, in accordance with local and national regulations.

Language

en

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