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A YY1-binding site is required for accurate human LINE-1 transcription initiation.

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posted on 2009-12-08, 16:12 authored by J .N. Athanikar, Richard M. Badge, J. V. Moran
The initial step in Long Interspersed Element-1 (LINE-1) retrotransposition requires transcription from an internal promoter located within its 5′-untranslated region (5′-UTR). Previous studies have identified a YY1 (Yin Yang 1)-binding site as an important sequence in LINE-1 transcription. Here, we demonstrate that mutations in the YY1-binding site have only minor effects on transcription activation of the full-length 5′-UTR and LINE-1 mobility in a single round cultured cell retrotransposition assay. Instead, these mutations disrupt proper initiation of transcription from the +1 site of the 5′-UTR. Thus, we propose that the YY1-binding site functions as a component of the LINE-1 core promoter to direct accurate transcription initiation. Indeed, this sequence may explain the evolutionary success of LINE-1 by enabling full-length retrotransposed copies to undergo autonomous retrotransposition in subsequent generations.

Funding

p. This work was supported by a grant from the National Institutes of Health (GM60518) awarded to J.V.M. J.N.A. was supported in part by a University of Michigan Cancer Biology postdoctoral training grant (CA009676) and then by a NRSA postdoctoral fellowship (F32GM20859). R.M.B. was supported by a Wellcome Trust Project Grant (069702/Z/02/Z).

History

Citation

Nucleic Acids Research, 2004, 32 (13), pp.3846-3855

Published in

Nucleic Acids Research

Publisher

Oxford University Press (OUP)

issn

1362-4962

eissn

1362-4962

Available date

2009-12-08

Publisher version

http://nar.oxfordjournals.org/content/32/13/3846

Language

en

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