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A high-resolution HAPPY map of Dictyostelium discoideum chromosome 6

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posted on 2012-10-24, 09:22 authored by B. A. Konfortov, H. M. Cohen, A. T. Bankier, P. H. Dear
We have made a high-resolution HAPPY map of chromosome 6 of Dictyostelium discoideum consisting of 300 sequence-tagged sites with an average spacing of 14 kb along the ∼4-Mb chromosome. The majority of the marker sequences were derived from randomly chosen clones from four different chromosome 6–enriched plasmid libraries or from subclones of YACs previously mapped to chromosome 6. The map appears to span the entire chromosome, although marker density is greater in some regions than in others and is lowest within the telomeric region. Our map largely supports previous gene-based maps of this chromosome but reveals a number of errors in the physical map. In addition, we find that a high proportion of the plasmid sequences derived from gel-enriched chromosome 6 (and that form the basis of a chromosome-specific sequencing project) originates from other chromosomes.

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Citation

Genome Research, 2000, 10 (11), pp. 1737-1742

Published in

Genome Research

Publisher

Cold Spring Harbor Laboratory Press

issn

1088-9051

Copyright date

2000

Available date

2012-10-24

Publisher version

http://genome.cshlp.org/content/10/11/1737

Language

en

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