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A simple method for the determination of reduction potentials in heme proteins

journal contribution
posted on 2014-03-21, 11:00 authored by Igor Efimov, Gary Parkin, Elizabeth S. Millett, Jennifer Glenday, Cheuk K. Chan, Holly Weedon, Harpreet Randhawa, Jaswir Basran, Emma L. Raven
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E[subscript m] = ˗115 mV ± 2 mV, pH 7.0) and human CLOCK (E[subscript m] = ˗111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.

Funding

This work was supported in part by Grants from BBSRC (Grant BB/C00602X/1 to E.R.) and The Wellcome Trust (083636 to E.R.).

History

Citation

FEBS Letters, 2014, 588 (5), pp. 701-704

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Biochemistry

Version

  • VoR (Version of Record)

Published in

FEBS Letters

Publisher

Elsevier on behalf of Federation of European Biochemical Societies

issn

0014-5793

eissn

1873-3468

Copyright date

2014

Available date

2014-03-21

Publisher version

http://www.sciencedirect.com/science/article/pii/S0014579314000283

Language

en

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