A simple method for the determination of reduction potentials in heme proteins
journal contributionposted on 2014-03-21, 11:00 authored by Igor Efimov, Gary Parkin, Elizabeth S. Millett, Jennifer Glenday, Cheuk K. Chan, Holly Weedon, Harpreet Randhawa, Jaswir Basran, Emma L. Raven
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E[subscript m] = ˗115 mV ± 2 mV, pH 7.0) and human CLOCK (E[subscript m] = ˗111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.
This work was supported in part by Grants from BBSRC (Grant BB/C00602X/1 to E.R.) and The Wellcome Trust (083636 to E.R.).
CitationFEBS Letters, 2014, 588 (5), pp. 701-704
Author affiliation/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Biochemistry
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