Adoption of a Turn Conformation Drives the Binding Affinity of p53 C-Terminal Domain Peptides to 14-3-3σ

The research is supported by funding from the European Union through the AEGIS project (H2020-MSCA-ITN-2015, Grant Number 67555), TASPPI project (H2020-MSCA-ITN2015, Grant Number 675179), and MSCA IEF (H2020- MSCA-IEF-2016, Grant Number 705188, R.G.D). J.F.N. and I.L. acknowledge LabEx (Laboratory of Excellence) for financial support on the scope of the DISTALZ consortium (ANR, ANR-11-LABX-009). E.S.-G. acknowledges a Plus-3 Grant of the Boehringer-Ingelheim Foundation and the computational time provided by the Computing and Data Facility of the Max Planck Society. The German Research Foundation (DFG) supported this work via the Collaborative Research Center SFB1093 (C.O., M.E., and E.S.-G.) and under Germany’s Excellence Strategy RESOLV EXC2033, Project Number 390677874 (E.S.-G., infrastructure support). We acknowledge F. X. Cantrelle for assistance on NMR data acquisition. The NMR facilities were funded by the Nord Region Council, CNRS, Institut Pasteur de Lille, the European Community (ERDF), and the French Ministry of Research and the University of Lille and by the CTRL CPER cofunded by the European Union with the European Regional Development Fund (ERDF), by the Hauts de France Regional Council (contract no. 17003781), Metropole Europe ́ enne de ́ Lille (contract no. 2016_ESR_05), and French State (contract no. 2017-R3-CTRL-Phase 1). We acknowledge support for the NMR facilities from TGE RMN THC (CNRS, FR-3050) and FRABio (Univ. Lille, CNRS, FR-3688).