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An HPLC-UV method for the simultaneous quantification of curcumin and its metabolites in plasma and lung tissue: potential for preclinical applications.

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posted on 2018-05-04, 10:54 authored by Lynne M. Howells, Jagdish Mahale, Robert G. Britton, Rajinder Singh, Hong Cai, Karen Brown
Curcumin, derived from turmeric, has been extensively investigated for its broad spectrum of biological 34 activities. Previously reported HPLC-UV methods have focussed on analysis of the parent compound. Here, a 35 sensitive HPLC-UV method was developed and partially validated, then used for the simultaneous 36 determination of curcumin and its glucuronide and sulfate metabolites in plasma and lung tissue from mice. The 37 assay was applied to an in vivo pharmacokinetic study comparing formulated curcumin (Meriva™) with 38 standard curcumin. Plasma levels of glucuronide and sulfate metabolites were 5-fold and 2-fold higher after 39 Meriva™ administration compared to standard curcumin. In lung tissue, free curcumin was 4-fold higher 40 following Meriva™ administration versus standard curcumin. This assay represents a rapid, cheap method for 41 simultaneous detection of curcumin and its major metabolites that has applicability in pre-clinical settings.

History

Citation

Biomedical Chromatography, 2018, 32(9), e4280

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Cancer Research Centre

Version

  • AM (Accepted Manuscript)

Published in

Biomedical Chromatography

Publisher

Wiley

issn

0269-3879

eissn

1099-0801

Copyright date

2018

Available date

2019-05-09

Publisher version

https://onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4280

Notes

The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.

Language

en

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