An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids
journal contributionposted on 2016-02-10, 14:48 authored by J. S. Amey, A. O. O'Reilly, Mark J. Burton, A. M. Puinean, I. R. Mellor, I. R. Duce, L. M. Field, B. A. Wallace, M. S. Williamson, T. G. E. Davies
We describe the identification in aphids of a unique heterodimeric voltage-gated sodium channel which has an atypical ion selectivity filter and, unusually for insect channels, is highly insensitive to tetrodotoxin. We demonstrate that this channel has most likely arisen by adaptation (gene fission or duplication) of an invertebrate ancestral mono(hetero)meric channel. This is the only identifiable voltage-gated sodium channel homologue in the aphid genome(s), and the channel's novel selectivity filter motif (DENS instead of the usual DEKA found in other eukaryotes) may result in a loss of sodium selectivity, as indicated experimentally in mutagenised Drosophila channels.
This work was funded by the U.K. Biotechnology and Biological Sciences Research Council (BBSRC) through grants BB/F007094/1(Rothamsted Research), BB/F009143/1 and BB/L006790 (Birkbeck College) and a Lawes Trust Studentship (Nottingham/Rothamsted).Funding for Myzus persicae genomic sequencing was provided by The Genome Analyses Centre (TGAC) Capacity and Capability Challenge programme project CCC-15 and BB/J004553/1 from the BBSRC and the John Innes Foundation.
CitationFEBS Letters, 2015, 589 (5), pp. 598-607
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