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Analysis of Meiotic Recombination Products from Human Sperm

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journal contribution
posted on 2010-06-24, 13:32 authored by Liisa Kauppi, Celia A. May, Alec J. Jeffreys
Traditional methods for surveying meiotic recombination in humans are limited to pedigree and linkage disequilibrium analyses. We have developed assays that allow the direct detection of crossover and gene conversion molecules in batches of sperm DNA. To date, we have characterized 26 recombination hotspots by allele-specific PCR and selectively amplified recombinant DNA molecules from these regions. These analyses have revealed that meiotic crossover hotspots in humans are highly localized and flanked by DNA segments where recombination is suppressed. The centers of crossover hotspots are also active in noncrossover recombination, displaying short conversion tracts.

History

Citation

Methods in Molecular Biology, 2009, 557, pp. 323-355.

Published in

Methods in Molecular Biology

Publisher

Humana Press

issn

1064-3745

isbn

9781934115664

Available date

2010-06-24

Publisher version

http://link.springer.com/protocol/10.1007/978-1-59745-527-5_20

Notes

This is the author's final draft of the paper published as Methods in Molecular Biology, 2009, 557, pp. 323-355. The original publication is available at www.springerlink.com. Doi: 10.1007/978-1-59745-527-5_20

Language

en

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