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Antibody to Plasmodium falciparum variant surface antigens, var gene transcription and ABO blood group in children with severe or uncomplicated malaria

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posted on 2024-02-13, 12:30 authored by Priyanka Barua, Michael F Duffy, Laurens Manning, Moses Laman, Timothy ME Davis, Ivo Mueller, Ali Haghiri, Julie A Simpson, James G Beeson, Stephen J Rogerson

Background

Antibodies to variant surface antigens (VSAs) such as Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) may vary with malaria severity. The influence of ABO blood group on antibody development is not understood.


Methods

Immunoglobulin G antibodies to VSAs in Papua New Guinean children with severe (n = 41) or uncomplicated (n = 30) malaria were measured by flow cytometry using homologous P falciparum isolates. Isolates were incubated with ABO-matched homologous and heterologous acute and convalescent plasma. RNA was used to assess var gene transcription.


Results

Antibodies to homologous, but not heterologous, isolates were boosted in convalescence. The relationship between antibody and severity varied by blood group. Antibodies to VSAs were similar in severe and uncomplicated malaria at presentation, higher in severe than uncomplicated malaria in convalescence, and higher in children with blood group O than other children. Six var gene transcripts best distinguished severe from uncomplicated malaria, including UpsA and 2 CIDRα1 domains.


Conclusions

ABO blood group may influence antibody acquisition to VSAs and susceptibility to severe malaria. Children in Papua New Guinea showed little evidence of acquisition of cross-reactive antibodies following malaria. Var gene transcripts in Papua New Guinean children with severe malaria were similar to those reported from Africa.

History

Author affiliation

School of Engineering, University of Leicester

Version

  • VoR (Version of Record)

Published in

The Journal of Infectious Diseases

Volume

228

Issue

8

Pagination

1099-1107

Publisher

Oxford University Press

Copyright date

2023

Available date

2024-02-13

Language

en

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