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Otero Fernandez 2019 Assessing the airborne survival of bacteria.pdf (1.16 MB)

Assessing the airborne survival of bacteria in populations of aerosol droplets with a novel technology

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journal contribution
posted on 2019-04-24, 13:29 authored by MO Fernandez, RJ Thomas, NJ Garton, A Hudson, A Haddrell, JP Reid
The airborne transmission of infection relies on the ability of pathogens to survive aerosol transport as they transit between hosts. Understanding the parameters that determine the survival of airborne microorganisms is critical to mitigating the impact of disease outbreaks. Conventional techniques for investigating bioaerosol longevity in vitro have systemic limitations that prevent the accurate representation of conditions that these particles would experience in the natural environment. Here, we report a new approach that enables the robust study of bioaerosol survival as a function of relevant environmental conditions. The methodology uses droplet-on-demand technology for the generation of bioaerosol droplets (1 to greater than 100 per trial) with tailored chemical and biological composition. These arrays of droplets are captured in an electrodynamic trap and levitated within a controlled environmental chamber. Droplets are then deposited on a substrate after a desired levitation period (less than 5 s to greater than 24 h). The response of bacteria to aerosolization can subsequently be determined by counting colony forming units, 24 h after deposition. In a first study, droplets formed from a suspension of Escherichia coli MRE162 cells (108 ml21 ) with initial radii of 27.8+0.08 mm were created and levitated for extended periods of time at 30% relative humidity. The time-dependence of the survival rate was measured over a time period extending to 1 h. We demonstrate that this approach can enable direct studies at the interface between aerobiology, atmospheric chemistry and aerosol physics to identify the factors that may affect the survival of airborne pathogens with the aim of developing infection control strategies for public health and biodefence applications.

Funding

The authors recognize the Natural Environment Research Council (NERC) and the Defence Science and Technology Laboratory (DSTL) for financial support through studentship funding.

History

Citation

Interface, 2019, 16 (150)

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Department of Infection, Immunity and Inflammation

Version

  • AM (Accepted Manuscript)

Published in

Interface

Publisher

The Royal Society

issn

1742-5689

eissn

1742-5662

Acceptance date

2018-12-17

Copyright date

2019

Available date

2019-04-24

Publisher version

https://royalsocietypublishing.org/doi/10.1098/rsif.2018.0779

Notes

Electronic supplementary material is available online at https://dx.doi.org/10.6084/m9.figshare.c.4347494

Language

en