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Beyond Saccharomyces pastorianus for modern lager brews: Exploring non-cerevisiae Saccharomyces hybrids with heterotic maltotriose consumption and novel aroma profile

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posted on 2023-03-10, 14:59 authored by Nikola Y Gyurchev, Angela Coral-Medina, Susan M Weening, Salwa Almayouf, Niels GA Kuijpers, Elke Nevoigt, Edward J Louis
Non-domesticated, wild Saccharomyces yeasts have promising characteristics for beer diversification, particularly when used in the generation of de novo interspecific hybrids. A major motivation for the current work was the question whether attractive novel Saccharomyces interspecific hybrids can be created for the production of exotic lager beers without using the genomic resources of the ale yeast Saccharomyces cerevisiae. Importantly, maltotriose utilization is an essential characteristic typically associated with domesticated ale/lager brewing strains. A high-throughput screening on nearly 200 strains representing all eight species of the Saccharomyces genus was conducted. Three Saccharomyces mikatae strains were able to aerobically grow on maltotriose as the sole carbon source, a trait until recently unidentified for this species. Our screening also confirmed the recently reported maltotriose utilization of the S. jurei strain D5095T. Remarkably, de novo hybrids between a maltotriose-utilizing S. mikatae or S. jurei strain and the maltotriose-negative Saccharomyces eubayanus strain CBS 12357T displayed heterosis and outperformed both parents with regard to aerobically utilizing maltotriose as the sole source of carbon. Indeed, the maximum specific growth rates on this sugar were comparable to the well-known industrial strain, Saccharomyces pastorianus CBS 1513. In lager brewing settings (oxygen-limited), the new hybrids were able to ferment maltose, while maltotriose was not metabolized. Favorable fruity esters were produced, demonstrating that the novel hybrids have the potential to add to the diversity of lager brewing.

Funding

The project was funded by the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie Grant Agreement No. 764927.

History

Citation

Gyurchev NY, Coral-Medina Á, Weening SM, Almayouf S, Kuijpers NGA, Nevoigt E and Louis EJ (2022) Beyond Saccharomyces pastorianus for modern lager brews: Exploring non-cerevisiae Saccharomyces hybrids with heterotic maltotriose consumption and novel aroma profile. Front. Microbiol. 13:1025132. doi: 10.3389/fmicb.2022.1025132

Author affiliation

Department of Genetics and Genome Biology

Version

  • VoR (Version of Record)

Published in

FRONTIERS IN MICROBIOLOGY

Volume

13

Pagination

1025132

Publisher

FRONTIERS MEDIA SA

issn

1664-302X

eissn

1664-302X

Acceptance date

2022-10-21

Copyright date

2022

Available date

2023-03-03

Language

English

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