University of Leicester
Browse

Blue-light photodegradation of ferricyanide under protein relevant conditions

Download (983.11 kB)
journal contribution
posted on 2025-04-16, 14:56 authored by Patrick DF Murton, Christiane R Timmel, Stuart R Mackenzie, Patricia Rodríguez-Maciá

Ferricyanide is commonly used as a reoxidant in photochemical studies of redox proteins including cytochromes, photosystem II and flavoproteins. A low-spin d5 complex, [Fe(III)(CN)6]3− is a powerful electron acceptor which efficiently reoxidises photo-generated radical species. Unfortunately, ferricyanide itself absorbs strongly in the blue and a better understanding of its own photochemistry is required. Here, we present a combined UV/Vis and infrared spectroscopic study of the blue light photo-induced degradation of ferricyanide under conditions commonly employed in photochemical studies of proteins. Clear differences are observed in the photochemistry in pure water, Tris buffer and 20% glycerol solution, which are interpreted in terms of solvent–ligand exchange and ligand to metal charge transfer. The implications for photochemical studies of proteins employing ferricyanide as a reoxidant are discussed.

History

Author affiliation

College of Science & Engineering Chemistry

Version

  • VoR (Version of Record)

Published in

Dalton Transactions

Volume

54

Issue

11

Pagination

4735 - 4742

Publisher

Royal Society of Chemistry (RSC)

issn

1477-9226

eissn

1477-9234

Copyright date

2025

Available date

2025-04-16

Spatial coverage

England

Language

en

Deposited by

Dr Patricia Rodriguez Macia

Deposit date

2025-04-11

Usage metrics

    University of Leicester Publications

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC