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Bradykinin-activated contractile signalling pathways in human myometrial cells are differentially regulated by arrestin proteins.

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journal contribution
posted on 2015-07-29, 14:52 authored by Jonathon Mark Willets, Paul J. Brighton, LN Windell, S Rana, Craig A. Nash, Justin Chi Konje
Bradykinin is associated with infections and inflammation, which given the strong correlation between uterine infection and preterm labour may imply that it could play a role in this process. Therefore, we investigated bradykinin signalling, and the roles that arrestin proteins play in their regulation in human myometrial cells. Bradykinin induced rapid, transient intracellular Ca[superscript: 2+] increases that were inhibited following B[subscript: 2] receptor (B[subscript: 2]R) antagonism. Arrestin2 or arrestin3 depletion enhanced and prolonged bradykinin-stimulated Ca[superscript: 2+] responses, and attenuated B[subscript: 2]R desensitisation. Knockdown of either arrestin enhanced B[subscript: 2]R-stimulated ERK1/2 signals. Moreover, depletion of either arrestin elevated peak-phase p38-MAPK signalling, yet only arrestin3 depletion prolonged B[subscript: 2]R-induced p38-MAPK signals. Arrestin2-knockdown augmented bradykinin-induced cell movement. Bradykinin stimulates pro-contractile signalling mechanisms in human myometrial cells and arrestin proteins play key roles in their regulation. Our data suggest bradykinin not only acts as an utertonin, but may also have the potential to enhance the contractile environment of the uterus.

History

Citation

Molecular and Cellular Endocrinology, 2015, 407, pp. 57-66

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Cancer Studies and Molecular Medicine

Version

  • AM (Accepted Manuscript)

Published in

Molecular and Cellular Endocrinology

Publisher

Elsevier

eissn

1872-8057

Acceptance date

2015-03-04

Copyright date

2015

Available date

2016-03-09

Publisher version

http://www.sciencedirect.com/science/article/pii/S030372071500115X

Language

en

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