Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection..pdf (2.01 MB)
Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
journal contributionposted on 2016-12-14, 16:49 authored by N. R. Lemoine, E. S. Mayall, T. Jones, D. Sheer, S. McDermid, P. Kendall-Taylor, D. Wynford-Thomas
Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.
This work was supported by a grant from the Welsh Scheme for the Development of Health Service Resources.
CitationBritish Journal of Cancer (1989) 60, 897–903.
Author affiliation/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine
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