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Chemical Constituents and Antidepressant-Like Effects in Ovariectomized Mice of the Ethanol Extract of Alternanthera philoxeroides

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posted on 2018-10-15, 14:21 authored by Charinya Khamphukdee, Orawan Monthakantirat, Yaowared Chulikhit, Suradet Buttachon, Michael Lee, Artur M. S. Silva, Nazim Sekeroglu, Anake Kijjoa
The previously unreported flavone glycoside, demethyltorosaflavone B (2) and the E-propenoic acid substituted flavone, torosaflavone E (3a), were isolated together with nine previously reported metabolites, including indole-3-carbaldehyde, oleanonic acid, vanillic acid, p-hydroxybenzoic acid, altheranthin (1a), alternanthin B (1b), demethyltorosaflavone D (3b), luteolin 8-C-E-propenoic acid (4) and chrysoeriol 7-O-rhamnoside (5), from the ethanol extract of the aerial part of Althernanthera philoxeroides. The crude ethanol extract was evaluated for its in vitro estrogenic activity in MCF-7 breast cancer cell line. The crude ethanol extract was also investigated in vivo for its antidepressant-like effects on ovariectomized mice using tail suspension and forced swimming tests, while its effect on the locomotor activity was evaluated by a Y-maze test. The effect of the crude extract on the serum corticosterone level, size and volume of uterus of the ovariectomized mice were also investigated. The expression of the mouse cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB), brain-derived neurotrophic factor (BDNF) and β-actin mRNAs in hippocampus and frontal cortex was also evaluated, using semiquantitative reverse transcription-polymerase chain reaction. The crude extract and the isolated compounds 1a, 1b, 3a, 3b and 5, were evaluated for their inhibitory effects on monoamine oxidases (MAOs)-A and -B.

Funding

This research was funded by the Thailand Research Fund (grant no.: RDG5520030).

History

Citation

Molecules, 2018, 23(9), 2202

Version

  • VoR (Version of Record)

Published in

Molecules

Publisher

MDPI

issn

1420-3049

eissn

1420-3049

Acceptance date

2018-08-29

Copyright date

2018

Available date

2018-10-15

Publisher version

https://www.mdpi.com/1420-3049/23/9/2202

Notes

The following are available online, Figure S1: Structures of indole-3-carbaldehyde, oleanonic acid, vanillic acid, p-hydroxybenzoic acid, isolated from the crude ethanol extract of Alternanthera philoxeroides (Mart.) Griseb, Figures S2–S29: 1D and 2D-NMR spectra of isolated compounds, Figure S30: Ortep view of althernanthin (1a).

Language

en

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