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Combinatorial screening of polymer nanoparticles for their ability to recognize epitopes of AAV-neutralizing antibodies

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posted on 2020-03-06, 13:22 authored by Elena Piletska, Eugeny Mirkes, Stanislav S Piletsky, Hasan Abosoglu, Alfeshani Cassim, Edmund Chu, Simon Doughty, Shaun-Jones Eganda, Hikari Fuchigami, Aleah Hussein, Meedhu Olickal, Neelay Parmar, Akhil Sebastian, Sergey A Piletsky
A library of 17 nanoparticles made of acrylate and methacrylate copolymers is prepared, characterized, and screened against six epitopes of adeno-associated viruses (AAV)-neutralizing antibodies to assess their affinity and specificity. Peptide epitopes are immobilized onto the surface of glass beads, packed in filtration microplates, and incubated with fluorescein-labelled nanoparticles. Following intense washing, the affinity of nanoparticles to immobilized epitopes is assessed by measuring the fluorescence of captured nanoparticles. The results show that polar monomers, acrylic acid in particular, have a positive impact on polymer affinity towards all peptides used in this study. The presence of hydrophobic monomers, on other hand, has a negative impact on polymer binding. The composition of peptides used in this study has no noticeable impact on the affinity of synthesized nanoparticles. The affinity of nanoparticles with the highest affinity to peptide targets does not exceed millimolar level. Overall, it is found that the synthesized library showed modest affinity but lacked specificity, which should be further “tuned,” for example, by using molecular imprinting to achieve an acceptable level of affinity and specificity for practical application.

History

Citation

J Mol Recognit. 2020;33:e2824

Author affiliation

School of Chemistry; School of Mathematics

Version

  • AM (Accepted Manuscript)

Published in

Journal of Molecular Recognition

Volume

33

Issue

4

Publisher

WILEY

issn

0952-3499

eissn

1099-1352

Acceptance date

2019-10-21

Copyright date

2019

Available date

2019-11-19

Publisher version

https://onlinelibrary.wiley.com/doi/full/10.1002/jmr.2824

Spatial coverage

England

Language

English

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