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Contrast enhanced micro-computed tomography resolves the 3-dimensional morphology of the cardiac conduction system in mammalian hearts.pdf (1.1 MB)

Contrast enhanced micro-computed tomography resolves the 3-dimensional morphology of the cardiac conduction system in mammalian hearts.

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posted on 2019-10-24, 14:44 authored by RS Stephenson, MR Boyett, G Hart, T Nikolaidou, X Cai, AF Corno, N Alphonso, N Jeffery, JC Jarvis
The general anatomy of the cardiac conduction system (CCS) has been known for 100 years, but its complex and irregular three-dimensional (3D) geometry is not so well understood. This is largely because the conducting tissue is not distinct from the surrounding tissue by dissection. The best descriptions of its anatomy come from studies based on serial sectioning of samples taken from the appropriate areas of the heart. Low X-ray attenuation has formerly ruled out micro-computed tomography (micro-CT) as a modality to resolve internal structures of soft tissue, but incorporation of iodine, which has a high molecular weight, into those tissues enhances the differential attenuation of X-rays and allows visualisation of fine detail in embryos and skeletal muscle. Here, with the use of a iodine based contrast agent (I(2)KI), we present contrast enhanced micro-CT images of cardiac tissue from rat and rabbit in which the three major subdivisions of the CCS can be differentiated from the surrounding contractile myocardium and visualised in 3D. Structures identified include the sinoatrial node (SAN) and the atrioventricular conduction axis: the penetrating bundle, His bundle, the bundle branches and the Purkinje network. Although the current findings are consistent with existing anatomical representations, the representations shown here offer superior resolution and are the first 3D representations of the CCS within a single intact mammalian heart.

Funding

Work was funded by the Cardiac Surgical Research Fund of the Alder Hey Childrens’ NHS Hospital Trust. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. All scanning was performed at the EPSRC-funded Henry Moseley X-Ray imaging Facility, Manchester University. We are grateful to the staff at the facility for their co-operation.

History

Citation

PLoS ONE, 2012, 7(4): e35299.

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Department of Cardiovascular Sciences

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  • VoR (Version of Record)

Published in

PLoS ONE

Publisher

Public Library of Science

eissn

1932-6203

Acceptance date

2012-03-14

Copyright date

2012

Available date

2019-10-24

Publisher version

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0035299

Notes

Correction 23 Apr 2012: Stephenson RS, Boyett MR, Hart G, Nikolaidou T, Cai X, et al. (2012) Correction: Contrast Enhanced Micro-Computed Tomography Resolves the 3-Dimensional Morphology of the Cardiac Conduction System in Mammalian Hearts. PLOS ONE 7(4): 10.1371/annotation/1baecd19-92b6-4683-b7d7-39c13a3f2e15. https://doi.org/10.1371/annotation/1baecd19-92b6-4683-b7d7-39c13a3f2e15 View correction

Language

en

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