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DASH-2: flexible, low-cost, and high-throughput SNP genotyping by dynamic allele-specific hybridization on membrane arrays

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posted on 2009-12-08, 16:12 authored by M. Jobs, W. M. Howell, L. Strömqvist, T. Mayr, Anthony J. Brookes
Genotyping technologies need to be continually improved in terms of their flexibility, cost-efficiency, and throughput, to push forward genome variation analysis. To this end, we have leveraged the inherent simplicity of dynamic allele-specific hybridization (DASH) and coupled it to recent innovations of centrifugal arrays and iFRET. We have thereby created a new genotyping platform we term DASH-2, which we demonstrate and evaluate in this report. The system is highly flexible in many ways (any plate format, PCR multiplexing, serial and parallel array processing, spectral-multiplexing of hybridization probes), thus supporting a wide range of application scales and objectives. Precision is demonstrated to be in the range 99.8–100%, and assay costs are 0.05 USD or less per genotype assignment. DASH-2 thus provides a powerful new alternative for genotyping practice, which can be used without the need for expensive robotics support.

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Citation

Genome Research, 2003, 13 (5), pp.916-924

Published in

Genome Research

Publisher

Cold Spring Harbor Laboratory Press

issn

1088-9051

Copyright date

2003

Available date

2009-12-08

Publisher version

http://genome.cshlp.org/content/13/5/916

Language

en

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