University of Leicester
Browse
1297-9716-42-65.pdf (315.23 kB)

Detection of prions in the faeces of sheep naturally infected with classical scrapie

Download (315.23 kB)
journal contribution
posted on 2015-07-01, 10:56 authored by C. Baker, L. Terry, L. Howells, K. Bishop, S. Everest, L. Thorne, B. C. Maddison, K. C. Gough
Classical scrapie is a naturally transmitted prion disease of sheep and goats. Contaminated environments may contribute to the spread of disease and evidence from animal models has implicated urine, blood, saliva, placenta and faeces as possible sources of the infection. Here we sought to determine whether sheep naturally infected with classical scrapie shed prions in their faeces. We used serial protein misfolding cyclic amplification (sPMCA) along with two extraction methods to examine faeces from sheep during both the clinical and preclinical phases of the disease and showed amplification of PrPSc in 7 of 15 and 14 of 14 sheep respectively. However PrPSc was not amplified from the faeces of 25 sheep not exposed to scrapie. These data represent the first demonstration of prion shedding in faeces from a naturally infected host and thus a likely source of prion contamination in the environment. Introduction

History

Citation

Veterinary Research, 2011, 42, p. 65

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Biology

Version

  • VoR (Version of Record)

Published in

Veterinary Research

Publisher

BioMed Central

issn

0928-4249

eissn

1297-9716

Copyright date

2011

Available date

2015-07-01

Publisher version

http://www.veterinaryresearch.org/content/42/1/65

Language

en

Usage metrics

    University of Leicester Publications

    Categories

    Keywords

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC