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Drosophila circadian rhythms in seminatural environments: Summer afternoon component is not an artifact and requires TrpA1 channels

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journal contribution
posted on 2019-03-27, 15:49 authored by EW Green, EK O'Callaghan, CN Hansen, S Bastianello, S Bhutani, S Vanin, JD Armstrong, R Costa, CP Kyriacou
Under standard laboratory conditions of rectangular light/dark cycles and constant warm temperature, Drosophila melanogaster show bursts of morning (M) and evening (E) locomotor activity and a "siesta" in the middle of the day. These M and E components have been critical for developing the neuronal dual oscillator model in which clock gene expression in key cells generates the circadian phenotype. However, under natural European summer conditions of cycling temperature and light intensity, an additional prominent afternoon (A) component that replaces the siesta is observed. This component has been described as an "artifact" of the TriKinetics locomotor monitoring system that is used by many circadian laboratories world wide. Using video recordings, we show that the A component is not an artifact, neither in the glass tubes used in TriKinetics monitors nor in open-field arenas. By studying various mutants in the visual and peripheral and internal thermo-sensitive pathways, we reveal that the M component is predominantly dependent on visual input, whereas the A component requires the internal thermo-sensitive channel transient receptor potential A1 (TrpA1). Knockdown of TrpA1 in different neuronal groups reveals that the reported expression of TrpA1 in clock neurons is unlikely to be involved in generating the summer locomotor profile, suggesting that other TrpA1 neurons are responsible for the A component. Studies of circadian rhythms under seminatural conditions therefore provide additional insights into the molecular basis of circadian entrainment that would otherwise be lost under the usual standard laboratory protocols.

Funding

E.W.G., C.N.H., and C.P.K. were supported by a Biotechnology and Biological Sciences Research Council grant, and E.K.O., C.P.K., R.C., and J.D.A. were supported by a Marie Curie Initial Training Network “INsecTime” award. S. Bhutani, S.V., C.P.K., and R.C. were supported by European Community Grant EUCLOCK.

History

Citation

Proc Natl Acad Sci U S A, 2015, 112 (28), pp. 8702-8707

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome Biology

Version

  • AM (Accepted Manuscript)

Published in

Proc Natl Acad Sci U S A

Publisher

National Academy of Sciences

eissn

1091-6490

Acceptance date

2015-06-05

Copyright date

2015

Available date

2019-03-27

Publisher version

https://www.pnas.org/content/112/28/8702

Language

en

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