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Fully integrated silicon probes for high-density recording of neural activity

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posted on 2018-03-16, 12:49 authored by J. J. Jun, N. A. Steinmetz, J. H. Siegle, D. J. Denman, M Bauza, B Barbarits, A. K. Lee, C. A. Anastassiou, A Andrei, Ç Aydın, M Barbic, T. J. Blanche, V Bonin, J Couto, B Dutta, S. L. Gratiy, D. A. Gutnisky, M Häusser, B Karsh, P Ledochowitsch, C. M. Lopez, C Mitelut, S Musa, Michael Okun, M Pachitariu, J Putzeys, P. D. Rich, C Rossant, W-L Sun, K Svoboda, M Carandini, K. D. Harris, C Koch, J O'Keefe, T. D. Harris
Sensory, motor and cognitive operations involve the coordinated action of large neuronal populations across multiple brain regions in both superficial and deep structures1,2. Existing extracellular probes record neural activity with excellent spatial and temporal (sub-millisecond) resolution, but from only a few dozen neurons per shank. Optical Ca2+ imaging3,4,5 offers more coverage but lacks the temporal resolution needed to distinguish individual spikes reliably and does not measure local field potentials. Until now, no technology compatible with use in unrestrained animals has combined high spatiotemporal resolution with large volume coverage. Here we design, fabricate and test a new silicon probe known as Neuropixels to meet this need. Each probe has 384 recording channels that can programmably address 960 complementary metal–oxide–semiconductor (CMOS) processing-compatible low-impedance TiN6 sites that tile a single 10-mm long, 70 × 20-μm cross-section shank. The 6 × 9-mm probe base is fabricated with the shank on a single chip. Voltage signals are filtered, amplified, multiplexed and digitized on the base, allowing the direct transmission of noise-free digital data from the probe. The combination of dense recording sites and high channel count yielded well-isolated spiking activity from hundreds of neurons per probe implanted in mice and rats. Using two probes, more than 700 well-isolated single neurons were recorded simultaneously from five brain structures in an awake mouse. The fully integrated functionality and small size of Neuropixels probes allowed large populations of neurons from several brain structures to be recorded in freely moving animals. This combination of high-performance electrode technology and scalable chip fabrication methods opens a path towards recording of brain-wide neural activity during behaviour.

History

Citation

Nature, 2017, 551, pp 232-236

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/MBSP Non-Medical Departments/Neuroscience, Psychology and Behaviour

Version

  • AM (Accepted Manuscript)

Published in

Nature

Publisher

Nature Publishing Group

issn

0028-0836

eissn

1476-4687

Acceptance date

2017-10-16

Copyright date

2017

Available date

2018-05-08

Publisher version

https://www.nature.com/articles/nature24636

Notes

The file associated with this record is under embargo until 6 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above

Language

en

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