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G protein-coupled receptor 30 regulates trophoblast invasion and its deficiency is associated with preeclampsia

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posted on 2016-10-10, 11:16 authored by Chao Tong, Xiang Feng, Jun Chen, Xingchen Qi, Liyuan Zhou, Shuming Shi, Kamana Kc, Joanna L. Stanley, Philip N. Baker, Hua Zhang
BACKGROUND: Preeclampsia is known to be associated with reduced circulating levels of estrogen. The effects of estrogen in preeclampsia are normally mediated by the classical estrogen receptors. Intriguingly, a novel estrogen receptor, G protein-coupled receptor 30 (GPR30), has been recently found to play an important role in several estrogenic effects. However, the mechanisms by which GPR30 may mediate the development of preeclampsia remain unknown. METHOD: We observed that the expression of GPR30 in placental trophoblast cells is lower in preeclamptic placentas compared with normotensive controls. We then investigated the role of GPR30 in trophoblast cell invasion by utilizing placental explants and the immortalized human trophoblast cell line (HTR8/SVneo). RESULTS: The selective GPR30 agonist G1 and a general estrogen receptors agonist 17-β-estradiol (E2) both improved trophoblast cells invasion by upregulating MMP9 expression and the PI3K-Akt signaling pathway. This effect was abolished by a selective GPR30 inhibitor G15, implying that GPR30 may be involved in regulating trophoblast invasion, and that down-regulation of this receptor may result in the development of preeclampsia. CONCLUSION: The present study suggests that GPR30 is a critical regulator of trophoblast cell invasion, and as such may be a potential therapeutic interventional target for preeclampsia and other pregnancy complications resulting from impaired trophoblast invasion.

History

Citation

Journal of Hypertension, 2016, 34 (4), pp. 710-718

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY

Version

  • AM (Accepted Manuscript)

Published in

Journal of Hypertension

Publisher

Wolters Kluwer Health, Inc.

issn

0263-6352

eissn

1473-5598

Acceptance date

2015-12-06

Copyright date

2016

Available date

2017-04-01

Publisher version

http://journals.lww.com/jhypertension/toc/2016/04000

Notes

Following the embargo period the above license applies.

Language

en

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