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HtrA2 deficiency causes mitochondrial uncoupling through the F₁F₀-ATP synthase and consequent ATP depletion.

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journal contribution
posted on 2013-11-14, 12:57 authored by H. Plun-Favreau, V.S. Burchell, K.M. Holmström, Z. Yao, E. Deas, K. Cain, V. Fedele, Nicoleta Moisoi, M. Campanella, L. Miguel Martins, N.W. Wood, A.V. Gourine, A.Y. Abramov
Loss of the mitochondrial protease HtrA2 (Omi) in mice leads to mitochondrial dysfunction, neurodegeneration and premature death, but the mechanism underlying this pathology remains unclear. Using primary cultures from wild-type and HtrA2-knockout mice, we find that HtrA2 deficiency significantly reduces mitochondrial membrane potential in a range of cell types. This depolarisation was found to result from mitochondrial uncoupling, as mitochondrial respiration was increased in HtrA2-deficient cells and respiratory control ratio was dramatically reduced. HtrA2-knockout cells exhibit increased proton translocation through the ATP synthase, in combination with decreased ATP production and truncation of the F1 α-subunit, suggesting the ATP synthase as the source of the proton leak. Uncoupling in the HtrA2-deficient mice is accompanied by altered breathing pattern and, on a cellular level, ATP depletion and vulnerability to chemical ischaemia. We propose that this vulnerability may ultimately cause the neurodegeneration observed in these mice.

History

Citation

Cell Death and Disease, 2012, 3, e335

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Cell Physiology and Pharmacology

Version

  • VoR (Version of Record)

Published in

Cell Death and Disease

Publisher

Nature Publishing Group for Associazione Differenziamento e Morte Cellulare

eissn

2041-4889

Copyright date

2012

Available date

2013-11-14

Publisher version

http://www.nature.com/cddis/journal/v3/n6/full/cddis201277a.html

Notes

PMCID: PMC3388244

Language

en