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IL-4 receptor dependent expansion of lung CD169+ macrophages in microfilaria-driven inflammation.pdf (7 MB)

IL-4 receptor dependent expansion of lung CD169+ macrophages in microfilaria-driven inflammation.

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posted on 2019-10-17, 10:13 authored by F Fercoq, E Remion, SJ Frohberger, N Vallarino-Lhermitte, A Hoerauf, J Le Quesne, F Landmann, MP Hübner, LM Carlin, C Martin
Lung disease is regularly reported in human filarial infections but the molecular pathogenesis of pulmonary filariasis is poorly understood. We used Litomosoides sigmodontis, a rodent filaria residing in the pleural cavity responsible for pleural inflammation, to model responses to human filarial infections and probe the mechanisms. Wild-type and Th2-deficient mice (ΔdblGata1 and Il-4receptor(r)a-/-/IL-5-/-) were infected with L. sigmodontis. Survival and growth of adult filariae and prevalence and density of microfilariae were evaluated. Cells and cytokines in the pleural cavity and bronchoalveolar space were characterized by imaging, flow cytometry and ELISA. Inflammatory pathways were evaluated by transcriptomic microarrays and lungs were isolated and analyzed for histopathological signatures. 40% of WT mice were amicrofilaremic whereas almost all mutant mice display blood microfilaremia. Microfilariae induced pleural, bronchoalveolar and lung-tissue inflammation associated with an increase in bronchoalveolar eosinophils and perivascular macrophages, production of mucus, visceral pleura alterations and fibrosis. Inflammation and pathology were decreased in Th2-deficient mice. An IL-4R-dependent increase of CD169 was observed on pleural and bronchoalveolar macrophages in microfilaremic mice. CD169+ tissue-resident macrophages were identified in the lungs with specific localizations. Strikingly, CD169+ macrophages increased significantly in the perivascular area in microfilaremic mice. These data describe lung inflammation and pathology in chronic filariasis and emphasize the role of Th2 responses according to the presence of microfilariae. It is also the first report implicating CD169+ lung macrophages in response to a Nematode infection.

Funding

CM is grateful for funding from the “Programme Procope Campus France” (35467XE) and from core funding from the Museum National d’Histoire Naturelle. FF and ER are recipients of a PhD fellowship from the “Ecole doctorale 227 (MNHN/UPMC)”. LMC is grateful for funding from, the Medical Research Council (MR/M01245X/1), Imperial College London, the NHLI Foundation, and core funding from Cancer Research UK; Microscopy was performed in the Imperial College Facility for Imaging by Light Microscopy (FILM) part supported by the Wellcome Trust (104931/Z/14/Z) and BBSRC (grant BB/L015129/1), and the Cancer Research UK Beatson Institute Beatson Advanced Imaging Resource (BAIR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

History

Citation

PLoS Neglected Tropical Diseases, 2019, 13(8): e0007691.

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Cancer Research Centre

Version

  • VoR (Version of Record)

Published in

PLoS Neglected Tropical Diseases

Publisher

Public Library of Science

eissn

1935-2735

Acceptance date

2019-08-06

Copyright date

2019

Available date

2019-10-17

Publisher version

https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007691

Notes

All relevant data are within the manuscript and its Supporting Information files.

Language

en

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