University of Leicester
Browse
1-s2.0-S0020751915002052-main.pdf (1.31 MB)

Identification and evaluation of vaccine candidate antigens from the poultry red mite (Dermanyssus gallinae)

Download (1.31 MB)
journal contribution
posted on 2016-02-18, 10:25 authored by K. Bartley, H. W. Wright, J. F. Huntley, E. D. T. Manson, N. F. Inglis, K. McLean, Mintu Nath, Y. Bartley, A. J. Nisbet
An aqueous extract of the haematophagous poultry ectoparasite, Dermanyssus gallinae, was subfractionated using anion exchange chromatography. Six of these subfractions were used to immunise hens and the blood from these hens was fed, in vitro, to poultry red mites. Mite mortality following these feeds was indicative of protective antigens in two of the subfractions, with the risks of mites dying being 3.1 and 3.7. times higher than in the control group (P <. 0.001). A combination of two-dimensional immunoblotting and immunoaffinity chromatography, using IgY from hens immunised with these subfractions, was used in concert with proteomic analyses to identify the strongest immunogenic proteins in each of these subfractions. Ten of the immunoreactive proteins were selected for assessment as vaccine candidates using the following criteria: intensity of immune recognition; likelihood of exposure of the antigen to the antibodies in a blood meal; proposed function and known vaccine potential of orthologous molecules. Recombinant versions of each of these 10 proteins were produced in Escherichia coli and were used to immunise hens. Subsequent in vitro feeding of mites on blood from these birds indicated that immunisation with Deg-SRP-1 (serpin), Deg-VIT-1 (vitellogenin), Deg-HGP-1 (hemelipoglycoprotein) or Deg-PUF-1 (a protein of unknown function) resulted in significantly increased risk of mite death (1.7-2.8. times higher than in mites fed blood from control hens immunised with adjuvant only, P <. 0.001). The potential for using these antigens in a recombinant vaccine is discussed.

History

Citation

International Journal for Parasitology, 2015

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Cardiovascular Sciences

Version

  • VoR (Version of Record)

Published in

International Journal for Parasitology

Publisher

Elsevier, Australian Society for Parasitology

issn

0020-7519

eissn

1879-0135

Acceptance date

2015-07-14

Copyright date

2015

Available date

2016-02-18

Publisher version

http://www.sciencedirect.com/science/article/pii/S0020751915002052

Language

en

Usage metrics

    University of Leicester Publications

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC