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Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants.
journal contributionposted on 2018-04-06, 15:26 authored by Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, Lynette Brownfield
The male germline of flowering plants develops within the vegetative cell of the male gametophyte and displays a distinct transcriptional profile. Key to understanding the development of this unique cell lineage is determining how gene expression is regulated within germline cells. This knowledge impacts upon our understanding of cell specification, differentiation, and plant fertility. Here, we describe methods to identify cis-regulatory modules (CRMs) that act as key regulatory regions in the promoters of germline-expressed genes. We detail the complimentary techniques of phylogenetic footprinting and the use of fluorescent reporters in pollen for the identification and verification of CRMs.
CitationMethods in Molecular Biology, 2017, 1669, pp. 275-293
Author affiliation/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Department of Genetics
- AM (Accepted Manuscript)