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Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants.

journal contribution
posted on 2018-04-06, 15:26 authored by Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, Lynette Brownfield
The male germline of flowering plants develops within the vegetative cell of the male gametophyte and displays a distinct transcriptional profile. Key to understanding the development of this unique cell lineage is determining how gene expression is regulated within germline cells. This knowledge impacts upon our understanding of cell specification, differentiation, and plant fertility. Here, we describe methods to identify cis-regulatory modules (CRMs) that act as key regulatory regions in the promoters of germline-expressed genes. We detail the complimentary techniques of phylogenetic footprinting and the use of fluorescent reporters in pollen for the identification and verification of CRMs.

History

Citation

Methods in Molecular Biology, 2017, 1669, pp. 275-293

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Department of Genetics

Version

  • AM (Accepted Manuscript)

Published in

Methods in Molecular Biology

Publisher

Humana Press (Springer Imprint)

issn

1064-3745

eissn

1940-6029

Copyright date

2017

Publisher version

https://link.springer.com/protocol/10.1007/978-1-4939-7286-9_22

Notes

The file associated with this record is under a permanent embargo in accordance with the publisher's policy. The full text may be available through the publisher links provided above.

Book series

Methods in Molecular Biology book series;1669

Language

en

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