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Immune stimulation reduces sleep and memory ability in Drosophila melanogaster.

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posted on 2015-04-22, 08:34 authored by Eamonn B. Mallon, A. Alghamdi, Robert T.K. Holdbrook, Ezio Rosato
Psychoneuroimmunology studies the increasing number of connections between neurobiology, immunology and behaviour. We demonstrate the effects of the immune response on two fundamental behaviours: sleep and memory ability in Drosophila melanogaster. We used the Geneswitch system to upregulate peptidoglycan receptor protein (PGRP) expression, thereby stimulating the immune system in the absence of infection. Geneswitch was activated by feeding the steroid RU486, to the flies. We used an aversive classical conditioning paradigm to quantify memory and measures of activity to infer sleep. Immune stimulated flies exhibited reduced levels of sleep, which could not be explained by a generalised increase in waking activity. Immune stimulated flies also showed a reduction in memory abilities. These results lend support to Drosophila as a model for immune-neural interactions and provide a possible role for sleep in the interplay between the immune response and memory.

Funding

ER and AA were funded by BBSRC grant BB/H018093/1 and a Saudi government scholarship respectively.

History

Citation

PeerJ, 2014, 2, pp. 2:e434;

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Genetics

Version

  • VoR (Version of Record)

Published in

PeerJ

Publisher

PeerJ

issn

2167-8359

eissn

2167-8359

Copyright date

2014

Available date

2015-04-22

Publisher version

https://peerj.com/articles/434/

Notes

PMCID: PMC4060034 Complete ANOVAs DOI: 10.7717/peerj.434/supp-1

Language

en

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