In vitro responsiveness to serum growth factors is inversely related to in vivo malignancy in human thyroid epithelial cells..pdf (727.95 kB)
Download fileIn vitro responsiveness to serum growth factors is inversely related to in vivo malignancy in human thyroid epithelial cells.
journal contribution
posted on 2016-12-15, 09:22 authored by T. P. Dawson, F. S. Wyllie, D. Wynford-ThomasWe have examined the proliferative response (DNA synthesis) of primary thyroid epithelial cultures to serum and a defined serum-substitute. These cultures were derived from normal human thyroid and from thyroid adenomas and carcinomas. All normal cultures showed a dose-dependent response, with a maximum 3H-thymidine labelling index of around 50%. Three out of the four adenomas demonstrated a much reduced or delayed response under the same conditions. In two carcinomas, labelling was never more than 5% and in one case was undetectable. This inverse relationship between the degree of in vivo malignancy and proliferative response in vitro has important implications for the interpretation of tissue culture models of epithelial neoplasia and also offers the potential for isolating novel growth factors specific for thyroid cancer cells.
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Citation
British Journal of Cancer, 1991, 63 (6), pp. 897-900Author affiliation
/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of MedicineVersion
- VoR (Version of Record)