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Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection.pdf (5.86 MB)

Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection

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posted on 2023-05-26, 13:54 authored by TK Nyazika, L Sibale, J Phiri, M De Ste Croix, Z Jasiunaite, C Mkandawire, R Malamba, A Kankwatira, M Manduwa, DM Ferreira, TS Nyirenda, MR Oggioni, HC Mwandumba, KC Jambo
People Living with HIV (PLHIV) are at an increased risk of pneumococcal pneumonia than HIV-uninfected adults, but the reasons for this are still not well understood. We investigated whether alveolar macrophages (AM) mediated control of pneumococcal infection is impaired in PLHIV compared to HIV-uninfected adults. We assessed anti-bactericidal activity against Streptococcus pneumoniae of primary human AM obtained from PLHIV and HIV-uninfected adults. We found that pneumococcus survived intracellularly in AMs at least 24 hours post ex vivo infection, and this was more frequent in PLHIV than HIV-uninfected adults. Corroborating these findings, in vivo evidence showed that PLHIV had a higher propensity for harboring S. pneumoniae within their AMs than HIV-uninfected adults. Moreover, bacterial intracellular survival in AMs was associated with extracellular propagation of pneumococcal infection. Our data suggest that failure of AMs to eliminate S. pneumoniae intracellularly could contribute to the increased risk of pneumococcal pneumonia in PLHIV.

Funding

KJ was supported by the Wellcome (UK) through an Intermediate Fellowship 105831/Z/14/Z. TiN was supported by a training grant awarded as part of the Wellcome Strategic award to Malawi-Liverpool-Wellcome Trust Clinical Research Programme 101113/Z/13/Z084 and the Legacy Award from the Federation of African Immunological Societies. HM was supported by the Medical Research Council (MRC, UK) through an African Research Leader award MR/PO20526/1. MC and MO were supported by an IDRF grant. MC was supported by a HIC-Vac training grant PS3187 and funds from the University of Leicester Doctoral College.

History

Citation

Nyazika TK, Sibale L, Phiri J, De Ste Croix M, Jasiunaite Z, Mkandawire C, Malamba R, Kankwatira A, Manduwa M, Ferreira DM, Nyirenda TS, Oggioni MR, Mwandumba HC and Jambo KC (2022) Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection. Front. Immunol. 13:992659. doi: 10.3389/fimmu.2022.992659

Author affiliation

Department of Genetics and Genome Biology

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  • VoR (Version of Record)

Published in

Frontiers in Immunology

Volume

13

Pagination

992659

Publisher

Frontiers Media SA

issn

1664-3224

eissn

1664-3224

Acceptance date

2022-08-31

Copyright date

2022

Available date

2023-05-26

Language

eng

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