University of Leicester
Browse
2020 Zamudio EnvMic.pdf (1.85 MB)

Lineage specific evolution and gene flow in Listeria monocytogenes is independent of bacteriophages.

Download (1.85 MB)
journal contribution
posted on 2020-07-22, 10:47 authored by Roxana Zamudio, Richard D Haigh, Joseph D Ralph, Megan De Ste Croix, Taurai Tasara, Katrin Zurfluh, Min Jung Kwun, Andrew D Millard, Stephen D Bentley, Nicolas J Croucher, Roger Stephan, Marco R Oggioni
Listeria monocytogenes is a foodborne pathogen causing systemic infection with high mortality. To allow efficient tracing of outbreaks a clear definition of the genomic signature of a cluster of related isolates is required, but lineage specific characteristics call for a more detailed understanding of evolution. In our work we used core genome MLST (cgMLST) to identify new outbreaks combined to core genome SNP analysis to characterize the population structure and gene flow between lineages. Whilst analysing differences between the four lineages of L. monocytogenes we have detected differences in the recombination rate, and interestingly also divergence in the SNP differences between sub-lineages. In addition, the exchange of core genome variation between the lineages exhibited a distinct pattern, with lineage III being the best donor for horizontal gene transfer. Whilst attempting to link bacteriophage mediated transduction to observed gene transfer, we found an inverse correlation between phage presence in a lineage and the extent of recombination. Irrespective of the profound differences in recombination rates observed between sub-lineages and lineages we found that the previously proposed cut-off of 10 allelic differences in cgMLST can be still considered valid for the definition of a foodborne outbreak cluster of L. monocytogenes.

Funding

Biotechnology and Biological Sciences Research Council. Grant Numbers: BB/N002903/1, BB/P504737/1 Wellcome Trust. Grant Number: 104169/Z/14/Z

History

Citation

Zamudio, R., Haigh, R.D., Ralph, J.D., De Ste Croix, M., Tasara, T., Zurfluh, K., Kwun, M.J., Millard, A.D., Bentley, S.D., Croucher, N.J., Stephan, R. and Oggioni, M.R. (2020), Lineageā€specific evolution and gene flow in Listeria monocytogenes are independent of bacteriophages. Environ Microbiol. doi:10.1111/1462-2920.15111

Version

  • VoR (Version of Record)

Published in

Environmental Microbiology

Publisher

Wiley for Society for Applied Microbiology (SfAM)

issn

1462-2912

eissn

1462-2920

Acceptance date

2020-05-30

Copyright date

2020

Available date

2020-07-22

Spatial coverage

England

Language

eng

Publisher version

https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.15111

Usage metrics

    University of Leicester Publications

    Categories

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC