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MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double Strand Break Repair in Rice

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journal contribution
posted on 2016-10-25, 14:36 authored by Yi He, Chong Wang, James D. Higgins, Junping Yu, Jie Zong, Pingli Lu, Dabing Zhang, Wanqi Liang
F-box proteins constitute a large superfamily in plants and play important roles in controlling many biological processes, but the roles of F-box proteins in male meiosis in plants remain unclear. Here, we identify the rice (Oryza sativa) F-box gene MEIOTIC F-BOX (MOF), which is essential for male meiotic progression. MOF belongs to the FBX subfamily and is predominantly active during leptotene to pachytene of prophase I. mof meiocytes display disrupted telomere bouquet formation, impaired pairing and synapsis of homologous chromosomes, and arrested meiocytes at late prophase I, followed by apoptosis. Although normal, programmed double-stranded DNA breaks (DSBs) form in mof mutants, foci of the phosphorylated histone variant γH2AX, a marker for DSBs, persist in the mutant, indicating that many of the DSBs remained unrepaired. The recruitment of Completion of meiosis I (COM1) and Radiation sensitive51C (RAD51C) to DSBs is severely compromised in mutant meiocytes, indicating that MOF is crucial for DSB end-processing and repair. Further analyses showed that MOF could physically interact with the rice SKP1-like Protein1 (OSK1), indicating that MOF functions as a component of the SCF E3 ligase to regulate meiotic progression in rice. Thus, this study reveals the essential role of an F-box protein in plant meiosis and provides helpful information for elucidating the roles of the ubiquitin proteasome system in plant meiotic progression.

History

Citation

The Plant Cell, 2016, 28 (8), pp. 1879-1893

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Department of Genetics

Version

  • AM (Accepted Manuscript)

Published in

The Plant Cell

Publisher

American Society of Plant Biologists

issn

1040-4651

eissn

1532-298X

Acceptance date

2016-07-18

Copyright date

2016

Available date

2016-10-25

Publisher version

http://www.plantcell.org/content/28/8/1879

Language

en

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