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MegaPlex PCR: a strategy for multiplex amplification

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journal contribution
posted on 2008-04-07, 12:28 authored by Linda Strömqvist Meuzelaar, Owen Lancaster, J. Paul Pasche, Guido Kopal, Anthony J. Brookes
‘MegaPlex PCR’ is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by coamplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.

History

Citation

Nature Methods, 2007, 4, pp.835-857

Published in

Nature Methods

Publisher

Nature Publishing Group

Available date

2008-04-07

Notes

This is the authors' final draft of the paper published as Nature Methods, 2007, 4, pp.835-837. The definitive published version is available from http://www.nature.com/nmeth/, doi:10.1038/nmeth1091

Language

en

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