University of Leicester
Browse
MouseBoneMarrow.pdf (265.54 kB)

Mouse Bone Marrow and Peripheral Blood Erythroid Cell Counts are Regulated by Different Autosomal Genetic Loci

Download (265.54 kB)
journal contribution
posted on 2007-06-05, 10:12 authored by M. Jawad, G. Giotopoulos, S. Fitch, C. Cole, M. Plumb, Christopher J. Talbot
Erythropoiesis is under fine control and genetic loci that effect it are likely to be important in a range of conditions. To assess the relative contributions of different genetic loci to parameters of erythropoiesis we have measured RBC counts in the peripheral circulation and committed erythroid cells (RBC and small normoblasts) in the bone marrow in a cohort of (CBA/H x C57BL/6) F2 mice to map quantitative trait loci (QTL). Candidate genes were assessed using bioinformatics and DNA sequencing. Different autosomal loci affect bone marrow (chromosomes 5, 11, and 19) and peripheral blood (chromosome 4) erythroid cell counts but there may be a common chromosome X locus. Spleen weight QTL were found on chromosomes 3, 15 and 17. Surprisingly, erythropoietin (EPO) is the best candidate quantitative trait gene (QTG) in the chromosome 5 locus that affects bone marrow but not peripheral blood erythroid cell counts. Epo gene expression is known to be genetically regulated in mice, but our data suggests a tissue-specific role for epo in mouse erythropoiesis that is also genetically determined. The identity of the other QTG will be important both to further knowledge of the control of erythropoiesis and as potential modifier genes for haematological disorders.

History

Citation

Blood Cells Molecules and Diseases, 2007, 38 (2), pp. 69-77.

Published in

Blood Cells Molecules and Diseases

Publisher

Elsevier

issn

1079-9796

Available date

2007-06-05

Publisher version

http://www.sciencedirect.com/science/article/pii/S1079979606002348

Notes

NOTICE: this is the author’s version of a work that was accepted for publication in Blood Cells Molecules and Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Blood Cells Molecules and Diseases, [38 (2), pp. 69-77 (2007)] 10.1016/j.bcmd.2006.10.009

Language

en

Usage metrics

    University of Leicester Publications

    Categories

    No categories selected

    Keywords

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC