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Mycobacterium tuberculosis RNA Polymerase-binding Protein A (RbpA) and Its Interactions with Sigma Factors

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journal contribution
posted on 2013-08-29, 14:44 authored by Alessio Bortoluzzi, Frederick W. Muskett, Lorna C. Waters, Philip W. Addis, Barbara Rieck, Thomas Munder, Susanne Schleier, Francesca Forti, Daniela Ghisotti, Mark D. Carr, Helen M. O'Hare
RNA polymerase-binding protein A (RbpA), encoded by Rv2050, is specific to the actinomycetes, where it is highly conserved. In the pathogen Mycobacterium tuberculosis, RbpA is essential for growth and survival. RbpA binds to the β subunit of the RNA polymerase where it activates transcription by unknown mechanisms, and it may also influence the response of M. tuberculosis to the current frontline anti-tuberculosis drug rifampicin. Here we report the solution structure of RbpA and identify the principle sigma factor σ[superscript A] and the stress-induced σ[superscript B] as interaction partners. The protein has a central ordered domain with a conserved hydrophobic surface that may be a potential protein interaction site. The N and C termini are highly dynamic and are involved in the interaction with the sigma factors. RbpA forms a tight complex with the N-terminal domain of σB via its N- and C-terminal regions. The interaction with sigma factors may explain how RbpA stabilizes sigma subunit binding to the core RNA polymerase and thereby promotes initiation complex formation. RbpA could therefore influence the competition between principal and alternative sigma factors and hence the transcription profile of the cell.

History

Citation

The Journal of Biological Chemistry, 2013, 288 (20), pp. 14438-14450

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammation

Version

  • AM (Accepted Manuscript)

Published in

The Journal of Biological Chemistry

Publisher

American Society for Biochemistry and Molecular Biology

issn

0021-9258

eissn

1083-351X

Copyright date

2013

Available date

2014-04-02

Publisher version

http://www.jbc.org/content/288/20/14438

Notes

This research was originally published in The Journal of Biological Chemistry. Bortoluzzi, A., Muskett, F.W. et al., Mycobacterium tuberculosis RNA Polymerase-binding Protein A (RbpA) and Its Interactions with Sigma Factors, The Journal of Biological Chemistry, 2013, 288 (20), pp. 14438-14450, © the American Society for Biochemistry and Molecular Biology.

Language

en

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