University of Leicester
Nucl. Acids Res.-2006-Dominguez-3634-45.pdf (3.58 MB)
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NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition

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journal contribution
posted on 2012-10-24, 09:21 authored by Cyril Dominguez, Frédéric H.-T. Allain
The heterogeneous nuclear ribonucleoprotein (hnRNP) F belongs to the hnRNP H family involved in the regulation of alternative splicing and polyadenylation and specifically recognizes poly(G) sequences (G-tracts). In particular, hnRNP F binds a G-tract of the Bcl-x RNA and regulates its alternative splicing, leading to two isoforms, Bcl-x[subscript S] and Bcl-x[subscript L], with antagonist functions. In order to gain insight into G-tract recognition by hnRNP H members, we initiated an NMR study of human hnRNP F. We present the solution structure of the three quasi RNA recognition motifs (qRRMs) of hnRNP F and identify the residues that are important for the interaction with the Bcl-x RNA by NMR chemical shift perturbation and mutagenesis experiments. The three qRRMs exhibit the canonical βαββαβ RRM fold but additional secondary structure elements are present in the two N-terminal qRRMs of hnRNP F. We show that qRRM1 and qRRM2 but not qRRM3 are responsible for G-tract recognition and that the residues of qRRM1 and qRRM2 involved in G-tract interaction are not on the β-sheet surface as observed for the classical RRM but are part of a short β-hairpin and two adjacent loops. These regions define a novel interaction surface for RNA recognition by RRMs.



Nucleic Acids Research, 2013, 34 (13), pp. 3634–3645


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