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Phenotypic Characterization and Comparative Genomic Analysis of Novel Salmonella Bacteriophages Isolated from a Tropical Rainforest

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posted on 2023-09-22, 10:31 authored by Prasanna Mutusamy, Kirnpal Kaur Banga Singh, Lee Su Yin, Bent Petersen, Thomas Sicheritz-Ponten, Martha RJ Clokie, Stella Loke, Andrew Millard, Sivachandran Parimannan, Heera Rajandas

Salmonella infections across the globe are becoming more challenging to control due to the emergence of multidrug-resistant (MDR) strains. Lytic phages may be suitable alternatives for treating these multidrug-resistant Salmonella infections. Most Salmonella phages to date were collected from human-impacted environments. To further explore the Salmonella phage space, and to potentially identify phages with novel characteristics, we characterized Salmonella-specific phages isolated from the Penang National Park, a conserved rainforest. Four phages with a broad lytic spectrum (kills >5 Salmonella serovars) were further characterized; they have isometric heads and cone-shaped tails, and genomes of ~39,900 bp, encoding 49 CDSs. As the genomes share a <95% sequence similarity to known genomes, the phages were classified as a new species within the genus Kayfunavirus. Interestingly, the phages displayed obvious differences in their lytic spectrum and pH stability, despite having a high sequence similarity (~99% ANI). Subsequent analysis revealed that the phages differed in the nucleotide sequence in the tail spike proteins, tail tubular proteins, and portal proteins, suggesting that the SNPs were responsible for their differing phenotypes. Our findings highlight the diversity of novel Salmonella bacteriophages from rainforest regions, which can be explored as an antimicrobial agent against MDR-Salmonella strains.

Funding

Program Konsortium Kecemerlangan Penyelidikan (JPT(BKPI)1000/016/018/25 (60)), provided by the Ministry of Higher Education of Malaysia

History

Author affiliation

Department of Genetics and Genome Biology, University of Leicester

Version

  • VoR (Version of Record)

Published in

International journal of molecular sciences

Volume

24

Issue

4

Pagination

3678

Publisher

MDPI AG

issn

1422-0067

eissn

1422-0067

Copyright date

2023

Available date

2023-09-22

Spatial coverage

Switzerland

Language

eng

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