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'Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China'.pdf (3.5 MB)

Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China

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posted on 2021-03-04, 17:54 authored by W Cong, M-Y Li, Y Zou, J-Y Ma, Bo Wang, Z-Y Jiang, HM Elsheikha
We conducted a large-scale epidemiological investigation to detect the prevalence of Toxoplasma gondii in four marine bivalve shellfish species collected from six representative coastal regions of Weihai, eastern China. Between January 2018 and December 2018, 14,535 marine bivalve shellfish pooled into 2907 samples were randomly collected and examined for T. gondii DNA by a nested PCR assay targeting B1 gene. The results showed that 2.8% (82) of the 2907 pooled samples were tested positive for T. gondii DNA. Two T. gondii genotype (ToxoDB Genotype #9 and ToxoDB Genotype #1) were identified PCR-restriction fragment length polymorphism analysis. Factors that were found significantly associated with the presence of T. gondii DNA in marine bivalve shellfish included the source of samples (being wild) (odds ratio [OR], 3.34; 95% confidence interval [CI], 2.00–5.84; p < 0.01), surface runoff near the sampling site (OR, 2.64; 95% CI, 1.47–4.72; p < 0.01), and presence of cats near the sampling site (OR, 1.77; 95% CI, 1.02–3.07; p = 0.04). Moreover, the prevalence of T. gondii DNA in marine bivalve shellfish correlated with temperature (Pearson’s correlation: R = 0.75, p = 0.0049) and precipitation (R = 0.87, p = 0.00021). These findings provide new insights into the presence of T. gondii DNA in marine bivalve shellfish and highlight the impact of human activity on marine pollution by such an important terrestrial pathogen pollutant.

Funding

National Natural Science Foundation of China (Grant No. 31702383), the Key Research and Development Program of Shandong Province, China (Grant no. 2019GSF108135) and the China Postdoctoral Science Foundation (Grant No. 2016M602145, 2019M652392).

History

Citation

Ecotoxicology and Environmental Safety Volume 213, 15 April 2021, 112048

Author affiliation

School of Mathematics & Actuarial Science

Version

  • VoR (Version of Record)

Published in

Ecotoxicology and Environmental Safety

Volume

213

Publisher

Elsevier

issn

0147-6513

Acceptance date

2021-02-08

Copyright date

2021

Available date

2021-02-19

Language

en

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