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Profiling cellular diversity in sponges informs animal cell type and nervous system evolution

journal contribution
posted on 2022-02-28, 12:17 authored by Jacob M Musser, Klaske J. Schippers, Michael Nickel, Giulia Mizzon, Andrea B. Kohn, Constantin Pape, Paolo Ronchi, Nikolaos Papadopoulos, Alexander J. Tarashansky, Jörg U Hammel, Florian Wolff, Cong Liang, Ana Hernández-Plaza, Carlos P. Cantalapiedra, Kaia Achim, Nicole L. Schieber, Leslie Pan, Fabian Ruperti, Warren R. Francis, Sergio Vargas, Svenja Kling, Maike Renkert, Maxim Polikarpov, Gleb Bourenkov, Roberto Feuda, Imre Gaspar, Pawel BurkhardtPawel Burkhardt, Bo Wang, Peer Bork, Martin Beck, Thomas R. Schneider, Anna Kreshuk, Gert Wörheide, Jaime Huerta-Cepas, Yannick Schwab, Leonid L. Moroz, Detlev Arendt
The evolutionary origin of metazoan cell types such as neurons and muscles is not known. Using whole-body single-cell RNA sequencing in a sponge, an animal without nervous system and musculature, we identified 18 distinct cell types. These include nitric oxide–sensitive contractile pinacocytes, amoeboid phagocytes, and secretory neuroid cells that reside in close contact with digestive choanocytes that express scaffolding and receptor proteins. Visualizing neuroid cells by correlative x-ray and electron microscopy revealed secretory vesicles and cellular projections enwrapping choanocyte microvilli and cilia. Our data show a communication system that is organized around sponge digestive chambers, using conserved modules that became incorporated into the pre- and postsynapse in the nervous systems of other animals.

Funding

National Institutes of Health: R01NS114491

H2020 Marie Skłodowska-Curie Actions

Marie Sklodowska-Curie Actions: No 764840

Human Frontier Science Program

National Science Foundation: 1146575

National Science Foundation: 1557923

National Science Foundation: 1548121

European Research Coucil BrainEvoDevo: 294810

European Research Coucil BrainEvoDevo: 788921

National Science Foundation: 1645219

Excellence Programme from the State Research Agency (AEI) of Spain: SEV-2016-0672

National Programme for Fostering Excellence in Scientific and Technical Research: PGC2018-098073-A-I00 MCIU/AEI/FEDER

Research Technical Support Staff Aid: PTA2019-017593-I / AEI / 10.13039/501100011033

History

Citation

SCIENCE, VOL. 374, NO. 6568, pp. 717-723 • DOI: 10.1126/science.abj2949

Author affiliation

Department of Genetics and Genome Biology, University of Leicester

Version

  • VoR (Version of Record)

Published in

Science

Volume

374

Issue

6568

Pagination

717 - 723

Publisher

American Association for the Advancement of Science

issn

0036-8075

eissn

1095-9203

Copyright date

2021

Language

English

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