University of Leicester
Browse
journal.ppat.1006339.pdf (8.36 MB)

Promiscuous signaling by a regulatory system unique to the pandemic PMEN1 pneumococcal lineage

Download (8.36 MB)
journal contribution
posted on 2017-07-07, 10:34 authored by Anagha Kadam, Rory A. Eutsey, Jason Rosch, Xinyu Miao, Mark Longwell, Wenjie Xu, Carol A. Woolford, Todd Hillman, Anfal Shakir Motib, Hasan Yesilkaya, Aaron P. l Mitchel, N. Luisa Hiller
Streptococcus pneumoniae (pneumococcus) is a leading cause of death and disease in children and elderly. Genetic variability among isolates from this species is high. These differences, often the product of gene loss or gene acquisition via horizontal gene transfer, can endow strains with new molecular pathways, diverse phenotypes, and ecological advantages. PMEN1 is a widespread and multidrug-resistant pneumococcal lineage. Using comparative genomics we have determined that a regulator-peptide signal transduction system, TprA2/PhrA2, was acquired by a PMEN1 ancestor and is encoded by the vast majority of strains in this lineage. We show that TprA2 is a negative regulator of a PMEN1-specific gene encoding a lanthionine-containing peptide (lcpA). The activity of TprA2 is modulated by its cognate peptide, PhrA2. Expression of phrA2 is density-dependent and its C-terminus relieves TprA2-mediated inhibition leading to expression of lcpA. In the pneumococcal mouse model with intranasal inoculation, TprA2 had no effect on nasopharyngeal colonization but was associated with decreased lung disease via its control of lcpA levels. Furthermore, the TprA2/PhrA2 system has integrated into the pneumococcal regulatory circuitry, as PhrA2 activates TprA/PhrA, a second regulator-peptide signal transduction system widespread among pneumococci. Extracellular PhrA2 can release TprA-mediated inhibition, activating expression of TprA-repressed genes in both PMEN1 cells as well as another pneumococcal lineage. Acquisition of TprA2/PhrA2 has provided PMEN1 isolates with a mechanism to promote commensalism over dissemination and control inter-strain gene regulation.

History

Citation

PLoS Pathogens, 2017, 13(5): e1006339

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammation

Version

  • VoR (Version of Record)

Published in

PLoS Pathogens

Publisher

Public Library of Science (PLoS)

issn

1553-7366

eissn

1553-7374

Acceptance date

2017-04-07

Copyright date

2017

Available date

2017-07-07

Publisher version

http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006339

Language

en

Usage metrics

    University of Leicester Publications

    Categories

    Keywords

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC