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Regulation of CD38 in proliferating chronic lymphocytic leukaemia cells stimulated with CD154 and IL-4

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posted on 2016-02-10, 09:37 authored by S. Willimott, M. Baou, S. Huf, Simon David Wagner
Background and Objectives Chronic lymphocytic leukemia (CLL) cells, like normal B-cells, exist in two populations in vivo : quiescent cells in the peripheral circulation and proliferating cells in lymph nodes. The surface marker CD38 has roles in cell adhesion and signaling. Its expres- sion correlates with poor clinical outcome and is associated with expression of the signaling intermediate ZAP-70, which is also a marker of poor prognosis. We investi- gated the regulation of CD38 and ZAP-70 in proliferating CLL cells. Design and Methods W e cultured CLL cells on a stromal cell la yer that maintains viability and also with some stromal cells expressing CD40 ligand (CD154) in order to measure changes in expression of CD38 and ZAP-70. Results W e demonstrated up-regulation of CD38 expression b y CD154. The degree of up-reg - ulation did not cor relate with clinical stage or mutational status. In addition in the majority of cases tested ZAP-70 expression increased in parallel with up-regulation of CD38 although discordant cases were also observed. Interpretation and Conclusions Overall w e demonstrated that regulation of CD38 in CLL is dynamic and dependent on signals from CD154 and a stromal cell la yer . W e speculate that CD38 and ZAP-70 are expressed in lymph node leukemic cells in both good and poor prognosis patients, but, in cases with good clinical outcome, these molecules are down-regulat- ed in the peripheral blood whereas in cases with poor prognosis their expression is maintained

History

Citation

Haematologica, 2007, 92, pp. 1359-1366

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Cancer Studies and Molecular Medicine

Version

  • VoR (Version of Record)

Published in

Haematologica

Publisher

Ferrata Storti Foundation with European Hematology Association

issn

0390-6078

eissn

1592-8721

Acceptance date

2007-07-23

Copyright date

2007

Available date

2016-02-10

Publisher version

http://www.haematologica.org/content/92/10/1359.short

Notes

Deposited with reference to the publisher’s archiving policy available on the SHERPA/RoMEO website.

Language

en

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