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Resonance Raman and UV-Visible Microscopy Reveals that Conditioning Red Blood Cells with Repeated Doses of Sodium Dithionite Increases Haemoglobin Oxygen Uptake

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posted on 2018-03-23, 14:28 authored by D Perez-Guaita, M de Veij, KM Marzec, ARD Almohammedi, D McNaughton, Andrew J. Hudson, BR Wood
Here we report that successive additions of fresh dithionite to a suspension of red blood cells (RBCs) increase the capacity of the cells to uptake oxygen. This effect was not observed when the RBCs were similarly preconditioned using gaseous N2 to induce short episodes of hypoxia. The effect of successive sodium dithionite and N2 gas additions on a population of functional erythrocytes was monitored using Raman confocal microscopy, with 514 nm excitation, and UV–visible microscopy. The results indicate that successive additions of sodium dithionite in a suspension of red blood cells leads to an increase in both the rate and the capacity of the RBCs to uptake oxygen. The sodium dithionite did not cause haemoglobin from lysed RBCs to uptake more oxygen after successive additions and hence this effect was only observed in functional intact RBCs. Experiments performed with polarised Raman spectroscopy suggest that sodium dithionite increases the disorder of Hb in the RBC facilitating oxygen diffusion.

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Citation

ChemistrySelect, 2017, 2 (11), pp. 3342-3346

Author affiliation

/Organisation/COLLEGE OF SCIENCE AND ENGINEERING/Department of Chemistry

Version

  • AM (Accepted Manuscript)

Published in

ChemistrySelect

Publisher

Wiley

issn

2365-6549

eissn

2365-6549

Acceptance date

2017-04-06

Copyright date

2017

Available date

2018-04-13

Publisher version

https://onlinelibrary.wiley.com/doi/abs/10.1002/slct.201700190

Notes

The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.

Language

en

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