Michniewski_et_al-2019-Environmental_Microbiology.pdf (4.91 MB)
Riding the wave of genomics to investigate aquatic coliphage diversity and activity.
journal contribution
posted on 2019-03-26, 09:41 authored by S Michniewski, T Redgwell, A Grigonyte, B Rihtman, M Aguilo-Ferretjans, J Christie-Oleza, E Jameson, DJ Scanlan, AD MillardBacteriophages infecting Escherichia coli (coliphages) have been used as a proxy for faecal matter and water quality from a variety of environments. However, the diversity of coliphages that are present in seawater remains largely unknown, with previous studies largely focusing on morphological diversity. Here, we isolated and characterised coliphages from three coastal locations in the UK and Poland. Comparative genomics and phylogenetic analysis of phage isolates facilitated the identification of putative new species within the genera Rb69virus and T5virus and a putative new genus within the subfamily Tunavirinae. Furthermore, genomic and proteomic analysis combined with host range analysis allowed the identification of putative tail fibre that is likely responsible for the observed differences in host range of phages vB_Eco_mar003J3 and vB_Eco_mar004NP2.
Funding
Bioinformatics analysis was carried out using MRC CLIMB Infrastructure MR/L015080/1. AM was funded by NERC AMR -EVAL FARMS (NE/N019881/1). T.R. and S.M. were in receipt of PhD studentships funded by the Natural Environment Research Council (NERC) CENTA DTP. A.G. was in receipt of a PhD studentship funded by the Engineering and Physical Sciences Research Council (ESPRC) SynBio.
History
Citation
Environmental Microbiology, 2019Author affiliation
/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Department of Infection, Immunity and InflammationVersion
- AM (Accepted Manuscript)