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Sensitivities of human monocytes and epithelial cells to pneumolysin are different
journal contribution
posted on 2007-11-29, 17:45 authored by Robert A. Hirst, Hasan Yesilkaya, Edwin Clitheroe, Andrew Rutman, Nicola Dufty, Timothy J. Mitchell, Christopher L. O'Callaghan, Peter W. AndrewThe Streptococcus pneumoniae pore-forming toxin, pneumolysin, is an important virulence factor in pneumococcal
pneumonia. The effect of pneumolysin on human lung epithelial and monocyte cell viability was compared. Pneumolysin caused a dose-dependent loss of viability of human lung epithelial (A549 and L132)
and monocyte (U937 and THP-1) cell lines. Analysis of the dose-response curves revealed similar log 50% inhibitory concentration (pIC50) values for A549, L132, and THP-1 of 0.12 +/-0.1, 0.02 +/-0.04, and 0.12 +/- 0.13 hemolytic units (HU), respectively, but U937 cells showed a significantly greater pIC50 of 0.42 +/- 0.12 HU. Differentiation of A549 and L132 with phorbol ester or THP-1 with gamma interferon had no effect on their sensitivity to pneumolysin. However, a significant decrease in the potency of pneumolysin against U937 cells followed gamma interferon treatment. The Hill slopes of the inhibition curves were greater than unity, indicating that pneumolysin may act with positive cooperativity. Analysis of pneumolysin-treated THP-1 cells by electron microscopy revealed membrane lesions of between 100 and 200 nm in diameter.
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Infection and Immunity, 2002, 70(2), pp.1017-1022Published in
Infection and ImmunityPublisher
American Society for MicrobiologyAvailable date
2007-11-29Notes
Copyright © 2002, American Society for Microbiology. Also available from the publisher's website at http://iai.asm.org/Language
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