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Sensor based on molecularly imprinted polymer membranes and smartphone for detection of Fusarium contamination in cereals

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posted on 2021-05-10, 09:20 authored by T Sergeyeva, D Yarynka, L Dubey, I Dubey, E Piletska, R Linnik, M Antonyuk, T Ternovska, O Brovko, S Piletsky, A El’skaya
The combination of the generic mobile technology and inherent stability, versatility and cost-effectiveness of the synthetic receptors allows producing optical sensors for potentially any analyte of interest, and, therefore, to qualify as a platform technology for a fast routine analysis of a large number of contaminated samples. To support this statement, we present here a novel miniature sensor based on a combination of molecularly imprinted polymer (MIP) membranes and a smartphone, which could be used for the point-of-care detection of an important food contaminant, oestrogen-like toxin zearalenone associated with Fusarium contamination of cereals. The detection is based on registration of natural fluorescence of zearalenone using a digital smartphone camera after it binds to the sensor recognition element. The recorded image is further processed using a mobile application. It shows here a first example of the zearalenone-specific MIP membranes synthesised in situ using “dummy template”-based approach with cyclododecyl 2, 4-dihydroxybenzoate as the template and 1-allylpiperazine as a functional monomer. The novel smartphone sensor system based on optimized MIP membranes provides zearalenone detection in cereal samples within the range of 1–10 µg mL−1 demonstrating a detection limit of 1 µg mL−1 in a direct sensing mode. In order to reach the level of sensitivity required for practical application, a competitive sensing mode is also developed. It is based on application of a highly-fluorescent structural analogue of zearalenone (2-[(pyrene-l-carbonyl) amino]ethyl 2,4-dihydroxybenzoate) which is capable to compete with the target mycotoxin for the binding to zearalenone-selective sites in the membrane’s structure. The competitive mode increases 100 times the sensor’s sensitivity and allows detecting zearalenone at 10 ng mL−1. The linear dynamic range in this case comprised 10–100 ng mL−1. The sensor system is tested and found effective for zearalenone detection in maize, wheat and rye flour samples both spiked and naturally contaminated. The developed MIP membrane-based smartphone sensor system is an example of a novel, inexpensive tool for food quality analysis, which is portable and can be used for the “field” measurements and easily translated into the practice.

Funding

Financial support from the National Academy of Sciences of Ukraine (project 0118U005176) and Ministry of Science and Education of Ukraine (project 0117U004234) is gratefully acknowledged.

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Citation

Sergeyeva, T.; Yarynka, D.; Dubey, L.; Dubey, I.; Piletska, E.; Linnik, R.; Antonyuk, M.; Ternovska, T.; Brovko, O.; Piletsky, S.; El’skaya, A. Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals. Sensors 2020, 20, 4304. https://doi.org/10.3390/s20154304

Version

  • VoR (Version of Record)

Published in

Sensors

Volume

20

Issue

15

Pagination

4304

Publisher

MDPI

issn

1424-8220

eissn

1424-8220

Acceptance date

2020-07-30

Copyright date

2020

Available date

2021-05-10

Spatial coverage

Switzerland

Language

English

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