Simple High-Resolution NMR Spectroscopy as A Tool in Molecular Biology

NMR is one of the major techniques for investigating the structure, dynamics and interactions between biomolecules. However, non‐experts often experience NMR experimentation and data analysis as intimidating. We discuss a simple yet powerful NMR technique, the so‐called chemical shift perturbation (CSP) analysis, as a tool to elucidate macromolecular interactions in small‐ and medium‐sized complexes, including protein‐protein, protein‐drug, and protein‐DNA/RNA interactions. We discuss current software packages for NMR data analysis and present a new interactive graphical tool implemented in CcpNmr AnalysisAssign version‐3, which can drastically reduce the time required for the CSP analysis. Lastly, we illustrate the usefulness of a protein three‐dimensional structure for interpretation of the CSP data.