J. Cell Biol. 2011 Lippi.pdf (3.99 MB)
Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology
journal contribution
posted on 2014-09-24, 15:15 authored by Giordano Lippi, Joern R. Steinert, Emma L. Marczylo, Sabina D'Oro, Roberto Fiore, Ian D. Forsythe, Gerhard Schratt, Michele Zoli, Pierluigi Nicotera, Kenneth W. YoungPrevious studies have demonstrated that microribonucleic acids (miRs) are key regulators of protein expression in the brain and modulate dendritic spine morphology and synaptic activity. To identify novel miRs involved in neuronal plasticity, we exposed adult mice to chronic treatments with nicotine, cocaine, or amphetamine, which are psychoactive drugs that induce well-documented neuroadaptations. We observed brain region- and drug-specific changes in miR expression levels and identified miR-29a/b as regulators of synaptic morphology. In vitro imaging experiments indicated that miR-29a/b reduce mushroom-shaped dendritic spines on hippocampal neurons with a concomitant increase in filopodial-like outgrowths, suggesting an effect on synapse formation via actin cytoskeleton remodeling. We identified Arpc3, a component of the ARP2/3 actin nucleation complex, as a bona fide target for down-regulation by miR-29a/b. This work provides evidence that targeting of Arpc3 by miR-29a/b fine tunes structural plasticity by regulating actin network branching in mature and developing spines.
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Citation
Journal of Cell Biology , 2011, 194 (6), pp. 889-904Author affiliation
/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Cell Physiology and PharmacologyVersion
- VoR (Version of Record)
Published in
Journal of Cell BiologyPublisher
Rockefeller University Pressissn
0021-9525eissn
1540-8140Copyright date
2011Available date
2014-09-24Publisher DOI
Publisher version
http://jcb.rupress.org/content/194/6/889Notes
PMCID: PMC3207289Language
enAdministrator link
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