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The 35S U5 snRNP Is Generated from the Activated Spliceosome during In vitro Splicing.

journal contribution
posted on 2015-06-12, 13:56 authored by Olga V. Makarova, Evgeny M. Makarov
Primary gene transcripts of eukaryotes contain introns, which are removed during processing by splicing machinery. Biochemical studies In vitro have identified a specific pathway in which introns are recognised and spliced out. This occurs by progressive formation of spliceosomal complexes designated as E, A, B, and C. The composition and structure of these spliceosomal conformations have been characterised in many detail. In contrast, transitions between the complexes and the intermediates of these reactions are currently less clear. We have previously isolated a novel 35S U5 snRNP from HeLa nuclear extracts. The protein composition of this particle differed from the canonical 20S U5 snRNPs but was remarkably similar to the activated B* spliceosomes. Based on this observation we have proposed a hypothesis that 35S U5 snRNPs represent a dissociation product of the spliceosome after both transesterification reactions are completed. Here we provide experimental evidence that 35S U5 snRNPs are generated from the activated B* spliceosomes during In vitro splicing.

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Citation

PLoS One, 2015, 10 (5) : e0128430

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Biochemistry

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  • VoR (Version of Record)

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PLoS One

Publisher

Public Library of Science

eissn

1932-6203

Copyright date

2015

Available date

2015-06-12

Publisher version

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0128430

Language

en

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