posted on 2019-08-15, 15:04authored byA Gonzalez-Magaña, A Ibáñez de Opakua, M Romano-Moreno, J Murciano-Calles, N Merino, I Luque, AL Rojas, S Onesti, FJ Blanco, A De Biasio
Human DNA polymerase δ is essential for DNA replication and acts in conjunction with the processivity factor proliferating cell nuclear antigen (PCNA). Besides its catalytic subunit (p125), pol δ comprises three regulatory subunits (p50, p68, and p12). PCNA interacts with all of these subunits, but only the interaction with p68 has been structurally characterized. Here, we report solution NMR-, isothermal titration calorimetry-, and X-ray crystallography-based analyses of the p12-PCNA interaction, which takes part in the modulation of the rate and fidelity of DNA synthesis by pol δ. We show that p12 binds with micromolar affinity to the classical PIP-binding pocket of PCNA via a highly atypical PIP-box located at the p12 N terminus. Unlike the canonical PIP-box of p68, the PIP-box of p12 lacks the conserved glutamine, binds through a 2-fork plug made of an isoleucine and a tyrosine residue at +3 and +8 positions, respectively, and is stabilized by an aspartate at +6 position, which creates a network of intra-molecular hydrogen bonds. These findings add to growing evidence that PCNA can bind a diverse range of protein sequences that may be broadly grouped as PIP-like motifs as has been previously suggested.
Funding
This work was supported by the Italian Association for Cancer Research (iCARE fellowship from AIRC and the European Commission to A. D. B. and AIRC Grant IG14718 to S. O.), by Grant CTQ2017-83810-R (MINECO/FEDER, UE; to F. J. B.), by MINECO Fellowship BES-2015-075847 (to A. G.-M.), and by Basque Government Predoctoral Fellowship PRE_2016_2_0249 (to M. R.-M.). The authors declare that they have no conflicts of interest with the contents of this article.
History
Citation
Journal of Biological Chemistry, 2019
Author affiliation
/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Molecular & Cell Biology
Version
VoR (Version of Record)
Published in
Journal of Biological Chemistry
Publisher
American Society for Biochemistry and Molecular Biology
The atomic coordinates and structure factors (code 6HVO) have been deposited in the Protein Data Bank (http://wwpdb.org/).
The NMR resonance data of this paper have been deposited in the Biological Magnetic Resonance Data Bank under BMRB accession codes 27661 and 27662, respectively.;The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.