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The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice.pdf (331.74 kB)

The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice.

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posted on 2019-10-02, 15:30 authored by D Chiavolini, G Memmi, T Maggi, F Iannelli, G Pozzi, MR Oggioni
BACKGROUND: Streptococcus pneumoniae possesses large zinc metalloproteinases on its surface. To analyse the importance in virulence of three of these metalloproteinases, intranasal challenge of MF1 outbred mice was carried out using a range of infecting doses of wild type and knock-out pneumococcal mutant strains, in order to compare mice survival. RESULTS: Observation of survival percentages over time and detection of LD50s of knock out mutants in the proteinase genes in comparison to the type 4 TIGR4 wild type strain revealed two major aspects: i) Iga and ZmpB, present in all strains of S. pneumoniae, strongly contribute to virulence in mice; (ii) ZmpC, only present in about 25% of pneumococcal strains, has a lower influence on virulence in mice. CONCLUSIONS: These data suggest Iga, ZmpB and ZmpC as candidate surface proteins responsible for pneumococcal infection and potentially involved in distinct stages of pneumococcal disease.

Funding

The work was supported in part by grants from Chiron Corporation (Emeryville, California), the Commission of the European Union (contract QLK2-2000-01536) and MIUR (COFIN 2002). The authors thank TIGR and Wellcome Trust Sanger Institute for sequences available over the web. Sequencing by TIGR of Streptococcus pneumoniae 670-6B was accomplished with support from NIAID / Univ. of Alabama, sequencing of Streptococcus gordonii was accomplished with support from TIGR and sequencing of Streptococcus mitis NCTC 12261 was accomplished with support from NIH-NIDCR. Sequencing by The Wellcome Trust Sanger Institute of Streptococcus pneumoniae Spanish 23F-1, in collaboration with Tim Mitchell and Peter Andrew, was accomplished with support from Beowulf Genomics.

History

Citation

BMC Microbiology, 2003, volume 3, Article number: 14

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Genetics and Genome Biology

Version

  • VoR (Version of Record)

Published in

BMC Microbiology

Publisher

BMC (part of Springer Nature)

eissn

1471-2180

Acceptance date

2003-07-03

Copyright date

2003

Available date

2019-10-02

Publisher version

https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-3-14

Language

en

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